Brought back into service for the Dominion War, she was assigned to the Second Fleet as a replacement vessel on stardate 51094.7.
The Tripoli participated in Operation Return. She suffered heavy damage and suffered high casualties during the battle.
She was later towed to Prophet's Landing to await repairs.
Captain Rivers, now in command of the USS Iroquois, sent an engineering team to the vessel to effect minor repairs during a stop at the planet in 2375.